Victor Alexander
USA
Title: Discovering of a reverse pathway in lymphocytes will change not only random theory of immune globulin synthesis and the central dogma of molecular biology, also will bring a novel super-antibody (sab) technology for very effective and fast treatment and cure of all of infectious diseases and cancer!
Biography
Biography: Victor Alexander
Abstract
Many unexplainable evidences in contemporary Molecular Immunology about Immune Globulin (Ig) synthesis (existence of high quantity of exons in V parts of Ig gene; formation of 2-4 palindromic (p-) sequences in L and H chains of Ig gene after 1st rearrangement; unexplainable “Hyper-mutation” phenomenon; localization of “Hyper-mutation” in the 3 CDRs (Complementarity-determining regions) of V chains; wasting of enormous quantity of new Lymphoid cells in B-Cell development is also evolutionary and scientifically unbelievable and allowed us to propose a New Conception about synthesis of Ig in B Lymphocytes as follows: 1. the 1st rearrangement of V segments of H and L chains in Pro-B and Pre-B lymphocytes in bone marrow is not a random, but a pattern rearrangement which is undergoing under toll-like receptor signaling through MSC to Pro- and Pre-B cells which are developing on the MSC. Choosing of particular V exon also isn’t a random event, but pattern rearrangement under particular toll-like receptor signaling. 2. affinity maturation of naïve B-cells is not a random event, but undergo by undiscovered yet Reverse Pathway mechanism, which passes exact genetic information about the best matching amino-acids (aa) chain to given antigen on MHC-2, through synthesis of short RNA in a special vesicle of cytoplasm of B-cells. 3. this nascent RNA which is carrying exact genetic information about high affine short amino-acid chain to given antigen, after connection with 2 steroid nuclear receptors moves to nucleus of B cell and connect with 2 palindromic sequences of V-(D)-J section of Ig gene, which are already formed after 1st pattern rearrangement and substitutes all of N nucleotides. And that way Ig gene is getting exact genetic information about the best matching (aa) to given antigen and transcribes high affinity m-RNA and consequently high affinity Ig. It is obvious that our new conception will change the central dogma of molecular biology and will create a novel, very effective and fast Super-Antibody (SAb) Technology for treatment and cure of all infectious diseases and cancer.