Shiju Chen
The First Affiliated Hospital of Xiamen University, China
Title: Altered expressed miR-920 in peripheral blood mononuclear cells of patient with acute gouty arthritis
Biography
Biography: Shiju Chen
Abstract
The acute gouty arthritis (GA) is one most painful acute inflammation induced by monosodium urate (MSU) deposition. The pathogenesis of inflammation remains unclear. Activation of MyD88/ NF-kB signal pathway is involved in acute GA, giving rise to the increase of cytokines including TNF-, IL-1, and IL-6. MicroRNAs(miRNAs), severing as post-transcriptional regulation, is reported to participate in many inflammatory diseases including acute GA. Previous study demonstrated miR-920 down-regulated in the peripheral white blood cells of GA which negative regulated target IL-1ß. This may be the role of miR-920 in regulating the production of proinflammatory cytokines in the pathogenesis of GA. To further explore the function of miR-920 in acute GA, we detected miR-920 by qRT-PCR in the peripheral blood mononuclear cells (PBMCs) of 9 acute gouty arthritis patients and 9 healthy controls. However, overexpression of miR-920 was found in PBMCs and MyD88 was predicated as another target gene by bioinformatics. After transfecting the miR-920 mimics or negative control mimics into human monocytic THP-1 cell line, expression of MyD88 decreased. This may hint an opposite role of miR-920 in acute gouty arthritis.